diff --git a/.github/workflows/tests.yml b/.github/workflows/tests.yml
index 65e1d3d..25dc581 100644
--- a/.github/workflows/tests.yml
+++ b/.github/workflows/tests.yml
@@ -39,7 +39,7 @@ jobs:
       run: |
         if [[ "$RUNNER_OS" == "Linux" ]]; then
           sudo apt-get update
-          sudo apt-get install -y build-essential zlib1g-dev
+          sudo apt-get install -y build-essential cmake zlib1g-dev
         fi
 
     - name: Setup conda (macOS only)
@@ -51,10 +51,15 @@ jobs:
         python-version: ${{ matrix.python-version }}
 
     - name: Install dependencies
+      env:
+        # Force pytantan to build from sdist so all Python versions get the
+        # same AVX2-off baseline. The published cp3x wheels have caused
+        # import-time failures on 3.9/3.10/3.11 in this matrix.
+        PIP_NO_BINARY: pytantan
       run: |
         if [[ "${{ matrix.use_conda }}" == "true" ]]; then
           # Use conda for macOS to avoid mappy build issues
-          conda install -c conda-forge -c bioconda mappy numpy pytest pytest-cov -y
+          conda install -c conda-forge -c bioconda mappy numpy pytest pytest-cov cmake -y
           # Install funannotate2 in editable mode - this will install all dependencies from pyproject.toml
           pip install -e .
         else
diff --git a/Dockerfile b/Dockerfile
index 03c6cb1..4a0ab29 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -4,7 +4,7 @@
 
 ARG PIXI_VERSION=0.67.0
 ARG UBUNTU_VERSION=22.04
-ARG PYTANTAN_VERSION=0.1.3
+ARG PYTANTAN_VERSION=0.1.4
 
 # ---------------------------------------------------------------------------
 # Stage 1: build — resolve + install the pixi environment from pixi.lock
diff --git a/pixi.lock b/pixi.lock
index 135aa3d..3c36f81 100644
--- a/pixi.lock
+++ b/pixi.lock
@@ -15,7 +15,6 @@ environments:
       - conda: https://conda.anaconda.org/conda-forge/noarch/aiohappyeyeballs-2.6.1-pyhd8ed1ab_0.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/aiohttp-3.13.5-py311h55b9665_0.conda
       - conda: https://conda.anaconda.org/conda-forge/noarch/aiosignal-1.4.0-pyhd8ed1ab_0.conda
-      - conda: https://conda.anaconda.org/conda-forge/noarch/archspec-0.2.5-pyhd8ed1ab_0.conda
       - conda: https://conda.anaconda.org/conda-forge/noarch/astunparse-1.6.3-pyhd8ed1ab_3.conda
       - conda: https://conda.anaconda.org/conda-forge/noarch/attrs-26.1.0-pyhcf101f3_0.conda
       - conda: https://conda.anaconda.org/bioconda/linux-64/augustus-3.5.0-pl5321h9716f88_9.conda
@@ -34,7 +33,7 @@ environments:
       - conda: https://conda.anaconda.org/conda-forge/noarch/blinker-1.9.0-pyhff2d567_0.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/boost-cpp-1.85.0-h44aadfe_2.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/brotli-python-1.2.0-py311h66f275b_1.conda
-      - conda: https://conda.anaconda.org/bioconda/noarch/buscolite-26.1.26-pyhdfd78af_0.conda
+      - conda: https://conda.anaconda.org/bioconda/noarch/buscolite-26.4.22-pyhdfd78af_0.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/bzip2-1.0.8-hda65f42_9.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/c-ares-1.34.6-hb03c661_0.conda
       - conda: https://conda.anaconda.org/conda-forge/noarch/ca-certificates-2026.2.25-hbd8a1cb_0.conda
@@ -185,7 +184,6 @@ environments:
       - conda: https://conda.anaconda.org/conda-forge/noarch/pyjwt-2.12.1-pyhcf101f3_0.conda
       - conda: https://conda.anaconda.org/conda-forge/noarch/pyopenssl-26.0.0-pyhcf101f3_0.conda
       - conda: https://conda.anaconda.org/conda-forge/noarch/pysocks-1.7.1-pyha55dd90_7.conda
-      - conda: https://conda.anaconda.org/bioconda/linux-64/pytantan-0.1.3-py311hc84137b_1.tar.bz2
       - conda: https://conda.anaconda.org/conda-forge/linux-64/python-3.11.15-hd63d673_0_cpython.conda
       - conda: https://conda.anaconda.org/bioconda/linux-64/python-edlib-1.3.9.post1-py311hf552afe_3.conda
       - conda: https://conda.anaconda.org/conda-forge/noarch/python-flatbuffers-25.9.23-pyh1e1bc0e_0.conda
@@ -198,7 +196,6 @@ environments:
       - conda: https://conda.anaconda.org/conda-forge/noarch/rsa-4.9.1-pyhd8ed1ab_0.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/s2n-1.7.1-h1cbb8d7_1.conda
       - conda: https://conda.anaconda.org/bioconda/linux-64/samtools-1.22.1-h96c455f_0.tar.bz2
-      - conda: https://conda.anaconda.org/bioconda/linux-64/scoring-matrices-0.3.4-py311haab0aaa_0.conda
       - conda: https://conda.anaconda.org/conda-forge/noarch/setuptools-82.0.1-pyh332efcf_0.conda
       - conda: https://conda.anaconda.org/conda-forge/noarch/six-1.17.0-pyhe01879c_1.conda
       - conda: https://conda.anaconda.org/bioconda/linux-64/snap-2017_03_01-h7b50bb2_0.tar.bz2
@@ -233,13 +230,16 @@ environments:
       - conda: https://conda.anaconda.org/conda-forge/noarch/zipp-3.23.1-pyhcf101f3_0.conda
       - conda: https://conda.anaconda.org/conda-forge/linux-64/zstd-1.5.7-hb78ec9c_6.conda
       - pypi: https://files.pythonhosted.org/packages/2c/2f/51d27f44689aa1ee000bb145811570002d420c7dfa0b153a992c28e0e297/annorefine-2026.2.22-cp311-cp311-manylinux_2_28_x86_64.whl
+      - pypi: https://files.pythonhosted.org/packages/d2/8d/2733707a9daf4df14cb6ae251691cfc49fcce888234ec6484364d8319b46/archspec-0.2.5-py3-none-any.whl
       - pypi: https://files.pythonhosted.org/packages/b1/89/2e3f29c41cb86273dcd8f3ec2df5b4e87ae0d554cbb268ae7c3092940cae/funannotate2_addons-26.3.7-py3-none-any.whl
       - pypi: https://files.pythonhosted.org/packages/31/f8/65d52c1383a1832761f80bad6206de6e9e675adb67aaa8673cc709fe84c2/helixerlite-25.5.27-cp311-cp311-manylinux_2_17_x86_64.manylinux2014_x86_64.whl
       - pypi: https://files.pythonhosted.org/packages/7b/91/984aca2ec129e2757d1e4e3c81c3fcda9d0f85b74670a094cc443d9ee949/joblib-1.5.3-py3-none-any.whl
       - pypi: https://files.pythonhosted.org/packages/9f/a0/b1266b0d852d10ac8339540d09fb90e38c198b6a2bcdeb3a881338d4a8fc/keras-layer-normalization-0.16.0.tar.gz
       - pypi: https://files.pythonhosted.org/packages/54/4b/195ac84cc8f6077b4f0f421e8daee21b7f1bd88cb7716414234379fe68ec/numcodecs-0.16.5-cp311-cp311-manylinux2014_x86_64.manylinux_2_17_x86_64.manylinux_2_28_x86_64.whl
+      - pypi: https://files.pythonhosted.org/packages/df/3e/6211c83f41a8d68e7002c615e8fa1b1da4d7a7a2c1fa0779f826871aa577/pytantan-0.1.4-cp311-abi3-manylinux_2_24_x86_64.manylinux_2_28_x86_64.whl
       - pypi: https://files.pythonhosted.org/packages/36/4d/4a67f30778a45d542bbea5db2dbfa1e9e100bf9ba64aefe34215ba9f11f6/scikit_learn-1.8.0-cp311-cp311-manylinux_2_27_x86_64.manylinux_2_28_x86_64.whl
       - pypi: https://files.pythonhosted.org/packages/09/7d/af933f0f6e0767995b4e2d705a0665e454d1c19402aa7e895de3951ebb04/scipy-1.17.1-cp311-cp311-manylinux_2_27_x86_64.manylinux_2_28_x86_64.whl
+      - pypi: https://files.pythonhosted.org/packages/1d/9c/9111e1e0fa82a8f48dd9d59853d7706cc3e04b01948fa6687e676fcf012d/scoring_matrices-0.3.4-cp311-abi3-manylinux1_x86_64.manylinux_2_28_x86_64.manylinux_2_5_x86_64.whl
       - pypi: https://files.pythonhosted.org/packages/24/94/80165946ec4986505cbfac29b5ae79544bfe2200d9d7883e1ad7c7342a55/tensorflow_addons-0.23.0-cp311-cp311-manylinux_2_17_x86_64.manylinux2014_x86_64.whl
       - pypi: https://files.pythonhosted.org/packages/32/d5/f9a850d79b0851d1d4ef6456097579a9005b31fea68726a4ae5f2d82ddd9/threadpoolctl-3.6.0-py3-none-any.whl
       - pypi: https://files.pythonhosted.org/packages/9a/bb/d43e5c75054e53efce310e79d63df0ac3f25e34c926be5dffb7d283fb2a8/typeguard-2.13.3-py3-none-any.whl
@@ -327,16 +327,11 @@ packages:
   - mypy ; extra == 'dev'
   - pre-commit ; extra == 'dev'
   requires_python: '>=3.9'
-- conda: https://conda.anaconda.org/conda-forge/noarch/archspec-0.2.5-pyhd8ed1ab_0.conda
-  sha256: eb68e1ce9e9a148168a4b1e257a8feebffdb0664b557bb526a1e4853f2d2fc00
-  md5: 845b38297fca2f2d18a29748e2ece7fa
-  depends:
-  - python >=3.9
-  license: MIT OR Apache-2.0
-  purls:
-  - pkg:pypi/archspec?source=hash-mapping
-  size: 50894
-  timestamp: 1737352715041
+- pypi: https://files.pythonhosted.org/packages/d2/8d/2733707a9daf4df14cb6ae251691cfc49fcce888234ec6484364d8319b46/archspec-0.2.5-py3-none-any.whl
+  name: archspec
+  version: 0.2.5
+  sha256: 604bd4115cb4c18e50a22a9b4a1e516706712263790d7d2994aaa595e70082f6
+  requires_python: '!=2.7.*,!=3.0.*,!=3.1.*,!=3.2.*,!=3.3.*,!=3.4.*,!=3.5.*,>=3.6'
 - conda: https://conda.anaconda.org/conda-forge/noarch/astunparse-1.6.3-pyhd8ed1ab_3.conda
   sha256: 7304f265f146235c34e24db310a94648aa306ca0b2a4a12042bf96da1881f99c
   md5: d3f195dfdbbf736e4ec178bbec2a975c
@@ -619,9 +614,9 @@ packages:
   - pkg:pypi/brotli?source=hash-mapping
   size: 367573
   timestamp: 1764017405384
-- conda: https://conda.anaconda.org/bioconda/noarch/buscolite-26.1.26-pyhdfd78af_0.conda
-  sha256: fc63cf6293135f48aab41f590ac0ec4ac4bd98aa81e2a845075cbb9847ab2050
-  md5: e286dc0c30c95ff9807edd94c7ccecb9
+- conda: https://conda.anaconda.org/bioconda/noarch/buscolite-26.4.22-pyhdfd78af_0.conda
+  sha256: e8e65402edf740ef445dde1f11afdb774626da4662dbe63e6d1d4df64b9bbe0d
+  md5: 87665555e8c53c63de12e64178e92f75
   depends:
   - augustus >=3.5.0
   - miniprot
@@ -634,8 +629,8 @@ packages:
   license_family: BSD
   purls:
   - pkg:pypi/buscolite?source=hash-mapping
-  size: 143758
-  timestamp: 1770583392184
+  size: 146187
+  timestamp: 1776846150347
 - conda: https://conda.anaconda.org/conda-forge/linux-64/bzip2-1.0.8-hda65f42_9.conda
   sha256: 0b75d45f0bba3e95dc693336fa51f40ea28c980131fec438afb7ce6118ed05f6
   md5: d2ffd7602c02f2b316fd921d39876885
@@ -808,21 +803,21 @@ packages:
   timestamp: 1752167340913
 - pypi: ./
   name: funannotate2
-  version: 26.2.12
-  sha256: 73ae21ea6a9a5edad01116a3a34a8d25b126e794283bd701ef650db129b93b6d
+  version: 26.4.22
+  sha256: 3433921df6b78d529c556af8edb76725db5a7abdfd6fdb1d0ea6cc41b30f7468
   requires_dist:
   - natsort
   - numpy
   - mappy
   - gfftk>=26.2.12
-  - buscolite>=26.1.26
+  - buscolite>=26.4.22
   - gapmm2>=25.8.12
   - pyhmmer>=0.12.0
   - pyfastx>=2.0.0
   - requests
   - gb-io>=0.3.2
   - json-repair
-  - pytantan
+  - pytantan>=0.1.4
   - psutil
   - annorefine>=2026.2.9
   requires_python: '>=3.9.0,<3.14'
@@ -2640,24 +2635,14 @@ packages:
   - pkg:pypi/pysocks?source=hash-mapping
   size: 21085
   timestamp: 1733217331982
-- conda: https://conda.anaconda.org/bioconda/linux-64/pytantan-0.1.3-py311hc84137b_1.tar.bz2
-  sha256: 6e39555316cd6aa55fe7980575c20ce498f99c9ae523c9a990bcf323febcdd52
-  md5: 1546f5c62ba6c422c4a827cb6b197f48
-  depends:
-  - archspec >=0.2
-  - libgcc >=13
-  - libstdcxx >=13
-  - libzlib >=1.3.1,<2.0a0
-  - python >=3.11,<3.12.0a0
-  - python_abi 3.11.* *_cp311
-  - scoring-matrices >=0.3.2
-  - scoring-matrices >=0.3.2,<0.4.0a0
-  license: GPL-3.0-or-later
-  license_family: GPL3
-  purls:
-  - pkg:pypi/pytantan?source=hash-mapping
-  size: 276866
-  timestamp: 1752626721522
+- pypi: https://files.pythonhosted.org/packages/df/3e/6211c83f41a8d68e7002c615e8fa1b1da4d7a7a2c1fa0779f826871aa577/pytantan-0.1.4-cp311-abi3-manylinux_2_24_x86_64.manylinux_2_28_x86_64.whl
+  name: pytantan
+  version: 0.1.4
+  sha256: 6273c806b2a3e106967136af616d55254263ffab225fd3e1147a3696e413a428
+  requires_dist:
+  - archspec~=0.2 ; os_name != 'nt'
+  - scoring-matrices~=0.3.0
+  requires_python: '>=3.7'
 - conda: https://conda.anaconda.org/conda-forge/linux-64/python-3.11.15-hd63d673_0_cpython.conda
   sha256: bf6a32c69889d38482436a786bea32276756cedf0e9805cc856ffd088e8d00f0
   md5: a5ebcefec0c12a333bcd6d7bf3bddc1f
@@ -2924,19 +2909,13 @@ packages:
   - ruff>=0.12.0 ; extra == 'dev'
   - cython-lint>=0.12.2 ; extra == 'dev'
   requires_python: '>=3.11'
-- conda: https://conda.anaconda.org/bioconda/linux-64/scoring-matrices-0.3.4-py311haab0aaa_0.conda
-  sha256: 83b017942cc60f23c31ebec6ef8c1be3f3f43f32c3e86223aba183406b2d9067
-  md5: 5c5703643ddc677df737cee6a1e931cd
-  depends:
-  - libgcc >=13
-  - python >=3.11,<3.12.0a0
-  - python_abi 3.11.* *_cp311
-  license: MIT
-  license_family: MIT
-  purls:
-  - pkg:pypi/scoring-matrices?source=hash-mapping
-  size: 113554
-  timestamp: 1768763699481
+- pypi: https://files.pythonhosted.org/packages/1d/9c/9111e1e0fa82a8f48dd9d59853d7706cc3e04b01948fa6687e676fcf012d/scoring_matrices-0.3.4-cp311-abi3-manylinux1_x86_64.manylinux_2_28_x86_64.manylinux_2_5_x86_64.whl
+  name: scoring-matrices
+  version: 0.3.4
+  sha256: fc0d2345e9b075eed40a1d9f3dca60053bb39232411d92b3d2d1c8536446e3bf
+  requires_dist:
+  - importlib-resources ; python_full_version < '3.9' and extra == 'test'
+  requires_python: '>=3.7'
 - conda: https://conda.anaconda.org/conda-forge/noarch/setuptools-82.0.1-pyh332efcf_0.conda
   sha256: 82088a6e4daa33329a30bc26dc19a98c7c1d3f05c0f73ce9845d4eab4924e9e1
   md5: 8e194e7b992f99a5015edbd4ebd38efd
diff --git a/pixi.toml b/pixi.toml
index c178636..62f36e8 100644
--- a/pixi.toml
+++ b/pixi.toml
@@ -23,11 +23,10 @@ table2asn = "*"
 # funannotate2 python deps available via bioconda / conda-forge
 gfftk = ">=26.2.12"
 gapmm2 = ">=25.8.12"
-buscolite = ">=26.1.26"
+buscolite = ">=26.4.22"
 pyhmmer = ">=0.12.0"
 pyfastx = ">=2.0.0"
 gb-io = ">=0.3.2"
-pytantan = "*"
 mappy = "*"
 
 # general python + scientific libs from conda-forge
@@ -50,4 +49,6 @@ funannotate2 = { path = ".", editable = false }
 "funannotate2-addons" = "*"
 helixerlite = "*"
 annorefine = ">=2026.2.9"
+# pytantan: bioconda lags at 0.1.3; pull 0.1.4+ from PyPI
+pytantan = ">=0.1.4"
 
diff --git a/pyproject.toml b/pyproject.toml
index c81f7bd..6238813 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -16,7 +16,7 @@ dependencies = [
     "numpy",
     "mappy",
     "gfftk>=26.2.12",
-    "buscolite>=26.2.22",
+    "buscolite>=26.4.22",
     "gapmm2>=25.8.12",
     "pyhmmer>=0.12.0",
     "pyfastx>=2.0.0",