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125 changes: 121 additions & 4 deletions _data/citations.yaml
Original file line number Diff line number Diff line change
@@ -1,5 +1,122 @@
# GENERATED AUTOMATICALLY, DO NOT EDIT

- id: https://arxiv.org/abs/2508.05800
title: Progress and new challenges in image-based profiling
authors:
- Erik Serrano
- John Peters
- Jesko Wagner
- Rebecca E. Graham
- Zhenghao Chen
- Brian Feng
- Gisele Miranda
- Alexandr A. Kalinin
- Loan Vulliard
- Jenna Tomkinson
- Cameron Mattson
- Michael J. Lippincott
- Ziqi Kang
- Divya Sitani
- Dave Bunten
- Srijit Seal
- Neil O. Carragher
- Anne E. Carpenter
- Shantanu Singh
- Paula A. Marin Zapata
- Juan C. Caicedo
- Gregory P. Way
publisher: arXiv
date: '2025-08-07'
link: http://arxiv.org/abs/2508.05800
image: https://arxiv.org/static/browse/0.3.4/images/arxiv-logo-one-color-white.svg
tags:
- image-based profiling
- review
- id: 10.1161/CIRCULATIONAHA.124.071956
title: Cell Painting and Machine Learning Distinguish Fibroblasts From Nonfailing
and Failing Human Hearts
authors:
- Joshua G. Travers
- Jenna Tomkinson
- Marcello Rubino
- Marion Delaunay
- Michael R. Bristow
- Gregory P. Way
- Timothy A. McKinsey
publisher: Circulation
date: '2025-04-22'
link: https://doi.org/g9wt6s
image: https://www.ahajournals.org/cms/10.1161/CIRCULATIONAHA.124.071956/asset/eb5df843-0afb-4f37-bf75-265f17c7ec93/assets/graphic/circulationaha.124.071956.fig01.jpg
tags:
- high-content microscopy
- cell painting
- cardiac fibrosis
extra-links:
- type: source
link: https://github.com/WayScience/cellpainting_predicts_cardiac_fibrosis
text: Analysis code
- id: 10.1091/mbc.E25-03-0119
title: A morphology and secretome map of pyroptosis
authors:
- Michael J. Lippincott
- Jenna Tomkinson
- Dave Bunten
- Milad Mohammadi
- Johanna Kastl
- Johannes Knop
- Ralf Schwandner
- Jiamin Huang
- Grant Ongo
- Nathaniel Robichaud
- Milad Dagher
- "Andr\xE9s Mansilla-Soto"
- Cynthia Saravia-Estrada
- Masafumi Tsuboi
- "Carla Basualto-Alarc\xF3n"
- Gregory P. Way
publisher: Molecular Biology of the Cell
date: '2025-06-01'
link: https://doi.org/g9wt6t
image: https://www.molbiolcell.org/cms/10.1091/mbc.E25-03-0119/asset/images/large/mbc-36-ar63-g001.jpeg
tags:
- cell death
- high-content microscopy
- multimodal data
extra-links:
- type: source
link: https://github.com/WayScience/pyroptosis_signature_image_profiling
text: Data processing
- type: source
link: https://github.com/WayScience/pyroptosis_signature_data_analysis
text: Analysis code
- id: 10.1038/s41467-025-60306-2
title: A versatile information retrieval framework for evaluating profile strength
and similarity
authors:
- Alexandr A. Kalinin
- John Arevalo
- Erik Serrano
- Loan Vulliard
- Hillary Tsang
- Michael Bornholdt
- "Al\xE1n F. Mu\xF1oz"
- Suganya Sivagurunathan
- Bartek Rajwa
- Anne E. Carpenter
- Gregory P. Way
- Shantanu Singh
publisher: Nature Communications
date: '2025-06-04'
link: https://doi.org/g9wt6v
image: https://www.biorxiv.org/content/biorxiv/early/2024/04/02/2024.04.01.587631/F1.large.jpg
tags:
- drug screening metric
- software
- profiling
extra-links:
- type: source
link: https://github.com/cytomining/copairs
text: Software
- id: 10.1038/s41592-025-02611-8
title: Reproducible image-based profiling with Pycytominer
authors:
Expand Down Expand Up @@ -41,7 +158,7 @@
- software
- cell morphology
extra-links:
- type: software
- type: source
link: https://github.com/cytomining/pycytominer
text: GitHub
- id: 10.1101/2024.09.11.612546
Expand All @@ -65,10 +182,10 @@
- cell morphology
- assay development
extra-links:
- type: software
- type: source
link: https://github.com/WayScience/nf1_schwann_cell_morphology_signature
text: Analysis code
- type: software
- type: source
link: https://github.com/WayScience/nf1_schwann_cell_painting_data
text: Data processing code
- id: 10.1038/s41592-024-02537-7
Expand Down Expand Up @@ -155,7 +272,7 @@
- type: data
link: https://github.com/WayScience/mitocheck_data
text: Data
- type: software
- type: source
link: https://github.com/WayScience/IDR_Stream
text: IDR_stream Software
- id: 10.1038/s41467-023-44045-w
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51 changes: 46 additions & 5 deletions _data/sources.yaml
Original file line number Diff line number Diff line change
@@ -1,3 +1,45 @@
- id: https://arxiv.org/abs/2508.05800
image: https://arxiv.org/static/browse/0.3.4/images/arxiv-logo-one-color-white.svg
tags:
- image-based profiling
- review

- id: 10.1161/CIRCULATIONAHA.124.071956
image: https://www.ahajournals.org/cms/10.1161/CIRCULATIONAHA.124.071956/asset/eb5df843-0afb-4f37-bf75-265f17c7ec93/assets/graphic/circulationaha.124.071956.fig01.jpg
tags:
- high-content microscopy
- cell painting
- cardiac fibrosis
extra-links:
- type: source
link: https://github.com/WayScience/cellpainting_predicts_cardiac_fibrosis
text: Analysis code

- id: 10.1091/mbc.E25-03-0119
image: https://www.molbiolcell.org/cms/10.1091/mbc.E25-03-0119/asset/images/large/mbc-36-ar63-g001.jpeg
tags:
- cell death
- high-content microscopy
- multimodal data
extra-links:
- type: source
link: https://github.com/WayScience/pyroptosis_signature_image_profiling
text: Data processing
- type: source
link: https://github.com/WayScience/pyroptosis_signature_data_analysis
text: Analysis code

- id: 10.1038/s41467-025-60306-2
image: https://www.biorxiv.org/content/biorxiv/early/2024/04/02/2024.04.01.587631/F1.large.jpg
tags:
- drug screening metric
- software
- profiling
extra-links:
- type: source
link: https://github.com/cytomining/copairs
text: Software

- id: 10.1038/s41592-025-02611-8
image: https://raw.githubusercontent.com/cytomining/pycytominer/master/logo/just-icon.png
tags:
Expand All @@ -6,7 +48,7 @@
- software
- cell morphology
extra-links:
- type: software
- type: source
link: https://github.com/cytomining/pycytominer
text: GitHub

Expand All @@ -18,13 +60,12 @@
- cell morphology
- assay development
extra-links:
- type: software
- type: source
link: https://github.com/WayScience/nf1_schwann_cell_morphology_signature
text: Analysis code
- type: software
- type: source
link: https://github.com/WayScience/nf1_schwann_cell_painting_data
text: Data processing code


- id: 10.1038/s41592-024-02537-7
image: https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fs41592-024-02537-7/MediaObjects/41592_2024_2537_Fig1_HTML.png
Expand Down Expand Up @@ -57,7 +98,7 @@
- type: data
link: https://github.com/WayScience/mitocheck_data
text: Data
- type: software
- type: source
link: https://github.com/WayScience/IDR_Stream
text: IDR_stream Software

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2 changes: 1 addition & 1 deletion research/index.md
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Expand Up @@ -13,7 +13,7 @@ We are specifically focused on the following work:

- **Reproducible software and methods for high-content microscopy analysis.** We are building open source software to support reproducible microscopy image analysis. We develop [pycytominer](https://github.com/cytomining/pycytominer), [CytoTable](https://github.com/cytomining/cytotable), and [coSMicQC](https://github.com/WayScience/cosmicqc) to analyze and process large-scale microscopy images. Our aim is to improve data processing pipelines, reproducibility, data provenance, and dataset interoperability. We are also innovating new methods and software for analyzing microscopy images across space and time.
- **Predicting cell phenotypes.** We extract biologically-meaningful and reproducible representations which contain information about cell phenotypes. We train artificial intelligence and machine learning (AI/ML) algorithms (e.g., virtual staining) to predict cell phenotypes and other markers from these images. These phenotypes include various cell health states, cell death mechanisms, and other important biological processes (e.g., nuclear speckles). Our aim is to use these representations to characterize and discover new biological processes and to annotate drug screening data with cell phenotypes.
- **Drug screening for pediatric diseases and cardiac fibrosis.** We perform microscopy-based, _in vitro_ drug screens to identify promising drug candidates. Our goal is to identify new therapeutic options for children with diseases like Neurofibromatosis Type 1 (NF1), neuroblastoma, and pediatric high grade glioma. We also have drug screening applications to discover innovative treatments for cardiac fibrosis.
- **Drug screening for pediatric cancers, Neurofibromatosis Type 1, and cardiac fibrosis.** We perform microscopy-based, _in vitro_ phenotypic drug screening to prioritize promising drug candidates and new targets. We are also developing patient-derived organoid phenotypic drug screening. Our goal is to identify new therapeutic strategies for diseases with urgent unmet needs.
- **Innovative method development for drug screening and translational research.** We develop new assays and computational methods to improve human health. This includes modeling NF1 and other pediatric diseases using patient-derived organoids, developing CRISPRi approaches to simulate specific high-dimensional phenotypes, modeling cell resistance to cancer therapies, and pioneering the concept we call "multi-gene dependencies", which we believe will revolutionize precision medicine for cancer patients.

## How we do science
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